苯基吡啶酮衍生物影响猪δ冠状病毒复制的作用分析

本研究旨在探讨苯基吡啶酮衍生物JIB-04对猪δ冠状病毒（porcine deltacoronavirus,PDCoV）的抗病毒作用,并初步研究可能的作用机制。首先应用CCK-8方法检测不同浓度JIB-04作用后的细胞活力,并计算半数毒性浓度（CC₅₀）和半数有效浓度（EC₅₀）。应用TCID₅₀方法检测JIB-04预处理和共处理对PDCoV复制的影响,检测JIB-04处理对PDCoV吸附或入侵细胞的影响。最后,应用qRT-PCR、TCID₅₀和Western blot方法检测JIB-04处理对不同时间点病毒复制的影响。结果表明,JIB-04在所有受试浓度下均不影响LLC-PK细胞的活力,CC₅₀>640 μmol·L^-1,EC₅₀=0.216 μmol·L^-1,SI指数>2 963。与未处理的病毒感染组相比,JIB-04处理极显著降低了PDCoV的滴度（P<0.001）,但对PDCoV吸附和入侵过程没有影响。感染6 h后,与未处理的病毒感染组相比,JIB-04处理组PDCoV滴度极显著下降（P<0.01）;感染12和24 h后,PDCoV滴度、基因组拷贝数、N蛋白表达水平均极显著下降（P<0.01）。JIB-04的细胞毒性较低,药物选择性指数较高,在体外能抵抗PDCoV感染,可以作为潜在的抗病毒药物。在PDCoV感染过程中,JIB-04能够抑制病毒核酸和蛋白质的合成,抑制病毒的复制。     

Initial shapes of stress-strain curve of wood specimen subjected to repeated combined compression and vibration stresses and the piezoelectric behavior

This study investigated the relationship between the initial shape of the stress (σ)-strain (ε) curve of a Chamaecyparis obtusa wood specimen subjected to repeated combined compression and vibration stresses at various angles between the fiber direction and load direction and the piezoelectric behavior. The main findings of the study are: (1) the σ-ε curve became convex initially, and then the stress was proportional to the strain. The σ-ε curve had almost the same shape during both loading and unloading. (2) The σ-piezoelectric voltage (P) curve was nonlinear, with a maximal point or cusp on the curve, which had almost the same shape during both loading and unloading, as was also observed for the σ-ε curve. (3) The plot of the first derivative of the stress [dσ/dε (= σ′)] against ε was nonlinear. The σ′-ε and P-ε curves at various angles were fairly similar. (4) The stress at the maximal point (or cusp) of the σ-P curve decreased with an increase in the angle between the fiber direction and load direction. The tendency of the stresses was very similar to that of Young’s modulus and compression strength calculated from Hook’s law and Hankinson’s law, respectively.     

中的鞘氨醇类代谢产物

从中国香港红树林的两株南海海洋真菌(2526#和1850#)的代谢产物中分离到4个化合物，通过完整的波谱数据分别解析为鞘氨醇类化合物N-(2’，3‘-二羟基二十四碳酰基)-1，3-二羟基-2-氨基十八烷(A)、n-(2’，3‘-二羟基二十六碳酰基)-1，3-二羟基-2-氨基十八烷(B)、2‘-dehydroxycerebroside D(C)和cerebroside D(D)。化合物A和B未见文献报道。     

云南海峰自然保护区旅游资源保护序位及开发

采用层次分析法,以各因子之间的相关性的高低代替专家赋值,分析云南海峰自然保护区的旅游资源.结果显示:在评价项目层中气象类重要值最高,水文类次之,接下去依次为植物类、地质地貌、生产用地、生活建筑和动物类;各评价项目层中起主要影响作用的因子分别为降水、地表径流、土壤、陆生植物、两栖类、蓄水池、防护林,其中降水、地表径流等水资源因子对湿地旅游资源的影响最为显著.根据研究结果,设计了几种不破坏景观资源的生态旅游活动,如观鸟旅游、观光农业旅游、民俗民居旅游、科考旅游和康体娱乐旅游等.     

宁蒙引黄灌区田间节水潜力计算方法分析

以黄河干流宁蒙大型自流引黄灌区为研究对象,采用分项法以及两种计算顺序对宁蒙引黄灌区田间节水潜力进行了计算,分析了不同计算顺序对田间节水潜力计算结果的影响,并采用综合法对分项法计算结果进行了验证,表明计算方法可靠。     

三阶开环控制系统根轨迹图形的研判

利用根轨迹极坐标方程对三阶系统根轨迹进行了研究，得出了三阶系统根轨迹的基本图形为双曲线的结论，并对图形的各种具体情况进行了进一步的讨论。     

The phytotoxicity of natural tetramic acid derivatives

Natural tetramic acid derivatives have attracted a great deal of interest of chemists because of their structural features and the broad range of biological activities. This paper focuses on five naturally occurring tetramic acid derivatives reported to have phytotoxicity and discusses their common structural characteristics. Copyright © 2011 Society of Chemical Industry     

农户经济增长与发展稳定性研究

通过大量长期跟踪观察的农户资料对农户纱济增长与发展进行了实证分析，揭示出农记脓庭经济增长与发展的稳定性是有条件的，在一定家庭生命周期段农户经济可以持续增长，当家庭生命周期运行到一定程度，农户经济增长必然出现衰退。     

Dietary protein requirements for discus (Symphysodon spp.)

Discus (Symphysodon spp.), a valuable ornamental species (mean initial weight 4.40–4.65 g) were fed five isoenergetic (gross energy: 200–209 kJ g^–1), semipurified diets (casein, gelatine and Danish fish meal as protein sources) twice a day to satiation for 12 weeks. Five levels of protein were evaluated (350, 400, 450, 500 and 550 g kg^–1 diet) and each fed to four replicates. Growth rate increased significantly with protein level up to 500 g kg^–1 diet and then decreased. Feed conversion rates (FCRs) ranged from 2.2 to 3.8, varying inversely with observed growth rate. A similar trend was also observed in the efficiency of protein utilization (PER). Analysis of dose (protein level)‐response (growth rate) with second order polynomial regression suggested a requirement of 449–501 g kg^–1.     

改良型UASB厌氧反应器研究进展

升流式厌氧污泥床反应器(Up-flow Anaerobic Sludge Blanket,UASB反应器)是一种较为先进的废水处理装置,被广泛用于畜禽养殖场有机废水处理。"十三五"以来,生态环境治理的新要求使得UASB反应器及相关技术愈加受到青睐。本文总结了UASB反应器的发展历程、整体结构、反应原理,同时还综合对比了常规UASB反应器与改良型UASB反应器的结构类型及其废水处理效果,并提出了UASB反应器未来的改进建议。